ZIC4 Antibody (PACO56082)
- SKU:
- PACO56082
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | ZIC4 Antibody (PACO56082) |
| Antibody SKU: | PACO56082 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Zinc finger protein ZIC 4 protein (28-88AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO56082 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of MCF-7 cells with PACO56082 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Binds to DNA. |
| Synonyms: | Zinc finger protein ZIC 4 (Zinc finger protein of the cerebellum 4), ZIC4 |
| UniProt Protein Function: | ZIC4: Binds to DNA. Belongs to the GLI C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 3q24 Cellular Component: nucleus Molecular Function:DNA binding |
| NCBI Summary: | This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009] |
| UniProt Code: | Q8N9L1 |
| NCBI GenInfo Identifier: | 85700440 |
| NCBI Gene ID: | 84107 |
| NCBI Accession: | Q8N9L1.3 |
| UniProt Secondary Accession: | Q8N9L1,Q4G157, Q9BZ94, A0AVA2, B2RMQ8, B4DF89, B7Z2L2 C9J7D5, J3KQG1, |
| UniProt Related Accession: | Q8N9L1 |
| Molecular Weight: | 40,641 Da |
| NCBI Full Name: | Zinc finger protein ZIC 4 |
| NCBI Synonym Full Names: | Zic family member 4 |
| NCBI Official Symbol: | ZIC4 |
| NCBI Protein Information: | zinc finger protein ZIC 4 |
| UniProt Protein Name: | Zinc finger protein ZIC 4 |
| UniProt Synonym Protein Names: | Zinc finger protein of the cerebellum 4 |
| Protein Family: | Zinc finger protein |
| UniProt Gene Name: | ZIC4 |
| UniProt Entry Name: | ZIC4_HUMAN |
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