XTT Cell Proliferation Assay Kit (CV0005)

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SKU:
CV0005
Product Type:
Assay
Research Area:
Cell Health
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Description

ELISA Kit Technical ManualMSDS

XTT Cell Proliferation Assay Kit

XTT Cell Proliferation Assay Kit is an optimized, accurate and sensitive colorimetric assay that detects the cellular metabolic activities. During the assay, the yellow tetrazolium salt XTT (sodium 2,3,-bis(2methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium) is reduced to a highly colored formazan dye by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of the formazan produced is proportional to viable cells in the sample.

The formazan dye formed in the assay is soluble in aqueous solution and quantified by measuring the absorbance at wavelength 450 nm using a spectrophotometer. An electron coupling reagent, such as PMS (N-Methylphenazonium methyl sulphate), can significantly improve the efficiency of XTT reduction in cells.

Features & Benefits

Overview Description

Size:

500/1000 Assays

Accurate:

Dye absorbance is proportional to the number of cells in each well.

Sensitive:

Assayed even in low cell concentrations.

Fast protocol:

Minimal handling steps & results within 2-5 hours, no solubilization step required.

Complete solution:

Includes all reagents needed for cell washing procedures.

Safe:

Non-radioactive.

Optimized:

Developed for high throughput assays (no washing or other steps that can cause cell loss and variability).

Applications:
  • Spectrophotometric quantification of cell proliferation and viability in response to pharmaceutical, chemical, nutrients and environmental compounds.
  • High Throughput Screening.

Storage & Shipping:
  • Shipped at: Ambient Temperature.
  • Storage: -20 °C.
  • Shelf Life: 24 months from date of purchase, if stored properly.
  • Recommendations: Protect XTT solution from light.

Buffers & Reagents:

Size

500 Assays:
  • 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent
  • 1 mL Activation Reagent

1000 Assays:
  • 1 x 25 mL XTT Cell Proliferation Assay Kit Reagent
  • 0.5 mL Activation Reagent

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