TRIM32 Antibody (PACO15150)
- SKU:
- PACO15150
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
| Antibody Name: | TRIM32 Antibody (PACO15150) |
| Antibody SKU: | PACO15150 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Fusion protein of human TRIM32 |
| Form: | Liquid |
| Storage Buffer: | -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol |
| Purification Method: | Antigen affinity purification |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO15150(TRIM32 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). |
 | Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Jurkat cells, PC3 cells, Lovo cells, Primary antibody: PACO15150(TRIM32 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds. |
 | The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO15150(TRIM32 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200). |
| Background: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. |
| Synonyms: | Tripartite motif containing 32 |
| UniProt Protein Function: | TRIM32: Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo. Defects in TRIM32 are the cause of limb-girdle muscular dystrophy type 2H (LGMD2H); also known as muscular dystrophy Hutterite type. LGMD2H is an autosomal recessive degenerative myopathy characterized by pelvic girdle, shoulder girdle and quadriceps muscle weakness. Clinical phenotype and severity are highly variable. Disease progression is slow and most patients remain ambulatory into the sixth decade of life. Defects in TRIM32 are the cause of Bardet-Biedl syndrome type 11 (BBS11). Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect. Belongs to the TRIM/RBCC family. |
| UniProt Protein Details: | Protein type:Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase Chromosomal Location of Human Ortholog: 9q33.1 Cellular Component: cytoplasm; striated muscle thick filament; cytosol; nucleus Molecular Function:protein binding; ubiquitin binding; protein self-association; myosin binding; zinc ion binding; Tat protein binding; RNA binding; translation initiation factor binding; transcription coactivator activity; ubiquitin-protein ligase activity; ligase activity Biological Process: fat cell differentiation; regulation of interferon type I production; positive regulation of neurogenesis; protein polyubiquitination; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of proteolysis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; positive regulation of cell cycle; positive regulation of cell growth; activation of NF-kappaB transcription factor; negative regulation of viral transcription; negative regulation of fibroblast proliferation; positive regulation of protein catabolic process; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity; positive regulation of neuron differentiation; response to UV; positive regulation of cell migration Disease: Muscular Dystrophy, Limb-girdle, Type 2h; Bardet-biedl Syndrome 11 |
| NCBI Summary: | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13049 |
| NCBI GenInfo Identifier: | 20178303 |
| NCBI Gene ID: | 22954 |
| NCBI Accession: | Q13049.2 |
| UniProt Secondary Accession: | Q13049,Q9NQP8, |
| UniProt Related Accession: | Q13049 |
| Molecular Weight: | 653 |
| NCBI Full Name: | E3 ubiquitin-protein ligase TRIM32 |
| NCBI Synonym Full Names: | tripartite motif containing 32 |
| NCBI Official Symbol: | TRIM32 |
| NCBI Official Synonym Symbols: | HT2A; BBS11; TATIP; LGMD2H |
| NCBI Protein Information: | E3 ubiquitin-protein ligase TRIM32; zinc finger protein HT2A; zinc-finger protein HT2A; 72 kDa Tat-interacting protein; TAT-interactive protein, 72-KD; tripartite motif-containing 32; tripartite motif-containing protein 32 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase TRIM32 |
| UniProt Synonym Protein Names: | 72 kDa Tat-interacting protein; Tripartite motif-containing protein 32; Zinc finger protein HT2A |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | TRIM32 |
| UniProt Entry Name: | TRI32_HUMAN |
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