TEAD1 Antibody (PACO43086)
- SKU:
- PACO43086
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | TEAD1 Antibody (PACO43086) |
| Antibody SKU: | PACO43086 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Transcriptional enhancer factor TEF-1 protein (135-215AA) |
| Form: | Liquid |
| Storage Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: TEAD1 antibody at 3.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 kDa. Observed band size: 48 kDa. |
 | Immunofluorescence staining of A549 cells with PACO43086 at 1:147, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO43086 diluted at 1:441 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. |
| Synonyms: | Transcriptional enhancer factor TEF-1 (NTEF-1) (Protein GT-IIC) (TEA domain family member 1) (TEAD-1) (Transcription factor 13) (TCF-13), TEAD1, TCF13 TEF1 |
| UniProt Protein Function: | TEAD1: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Interacts with YAP1 and WWTR1/TAZ. Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression Disease: Sveinsson Chorioretinal Atrophy |
| NCBI Summary: | This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010] |
| UniProt Code: | P28347 |
| NCBI GenInfo Identifier: | 3041733 |
| NCBI Gene ID: | 7003 |
| NCBI Accession: | P28347.2 |
| UniProt Secondary Accession: | P28347,A4FUP2, E7EV65, |
| UniProt Related Accession: | P28347 |
| Molecular Weight: | 426 |
| NCBI Full Name: | Transcriptional enhancer factor TEF-1 |
| NCBI Synonym Full Names: | TEA domain family member 1 (SV40 transcriptional enhancer factor) |
| NCBI Official Symbol: | TEAD1 |
| NCBI Official Synonym Symbols: | AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1 |
| NCBI Protein Information: | transcriptional enhancer factor TEF-1; protein GT-IIC; transcription factor 13; transcriptional enhancer factor 1 |
| UniProt Protein Name: | Transcriptional enhancer factor TEF-1 |
| UniProt Synonym Protein Names: | NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13 |
| Protein Family: | Transcriptional enhancer factor |
| UniProt Gene Name: | TEAD1 |
| UniProt Entry Name: | TEAD1_HUMAN |
| Antibodies |
| Anti-TEAD1 Antibody (CAB13366) |
| Anti-TEAD1 Antibody (CAB6768) |
| Anti-TEAD1 Antibody (CAB5218) |
| TEAD1 Antibody (PACO05189) |
| TEAD1 Antibody (PACO12690) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Anti-TEAD1 Antibody (CAB13366)
Human TEAD1 ELISA Kit (HUFI06164)
