T4 DNA Recombinant Protein (RPPB2404)

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SKU:
RPPB2404
Product type:
Recombinant Protein
Size:
100,000IU
Target:
T4 DNA
Synonyms:
DNA ligase 4
Synonyms:
EC 6511
Synonyms:
DNA ligase IV
Synonyms:
Polydeoxyribonucleotide synthase [ATP] 4
Source:
Escherichia Colilambda lysogen NM 989
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Description

Product Name:T4 DNA Recombinant Protein
Product Code:RPPB2404
Size:100,000IU
Target:T4 DNA
Synonyms:DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
Source:Escherichia Colilambda lysogen NM 989
Physical Appearance:Sterile filtered liquid formulation having a concentration of 167,000 U/ml.
Formulation:50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µm in 5´ termini). Optimal ligation occurs at 16C.
Biological Activity:One Weiss unit is equivalent to circa 67 cohesive-end ligation units.• T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.• Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50µM.• To dilute T4 DNA Ligase that will subsequently be stored at –20°C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

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