SSB (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit

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SKU:
CBCAB01476
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Reactivity:
Human
Detection Method:
Colorimetric
Frequently bought together:

Description

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SSB (Phospho-Ser366)Colorimetric Cell-Based ELISA Kit

The Human SSB (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit is carefully designed for the accurate detection of phosphorylated SSB (Phospho-Ser366) levels in cell lysates and tissue samples. This innovative kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Phosphorylated SSB (Phospho-Ser366) is a critical protein involved in DNA repair and maintenance, playing a crucial role in maintaining genomic stability.

Dysregulation of phosphorylated SSB levels has been linked to various diseases, including cancer, genetic disorders, and autoimmune conditions. Therefore, this ELISA kit serves as a valuable tool for researchers studying these conditions and developing potential therapeutic interventions.

Product Name:SSB (Phospho-Ser366) Colorimetric Cell-Based ELISA
Product Code:CBCAB01476
ELISA Type:Cell-Based
Target:SSB (Phospho-Ser366)
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nm
Format:2 x 96-Well Microplates

The SSB (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect SSB protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated SSB in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SSB phosphorylation.

Qualitative determination of SSB (Phospho-Ser366) concentration is achieved by an indirect ELISA format. In essence, SSB (Phospho-Ser366) is captured by SSB (Phospho-Ser366)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 6741, UniProt ID: P05455, OMIM: 109090, Unigene: Hs.632535
Gene Symbol:SSB
Sub Type:Phospho
UniProt Protein Function:SSB: a nuclear protein that plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5s, 7s, and 7-2 RNAs.
UniProt Protein Details:

Protein type:Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: nuclear chromosome, telomeric region; ribonucleoprotein complex

Molecular Function:mRNA binding; nucleotide binding; protein binding; tRNA binding

Biological Process: histone mRNA metabolic process; tRNA modification

NCBI Summary:The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]
UniProt Code:P05455
NCBI GenInfo Identifier:125985
NCBI Gene ID:6741
NCBI Accession:P05455.2
UniProt Secondary Accession:P05455,Q15367, Q53XJ4,
UniProt Related Accession:P05455
Molecular Weight:46,837 Da
NCBI Full Name:Lupus La protein
NCBI Synonym Full Names:Sjogren syndrome antigen B
NCBI Official Symbol:SSB  
NCBI Official Synonym Symbols:La; LARP3; La/SSB  
NCBI Protein Information:lupus La protein
UniProt Protein Name:Lupus La protein
UniProt Synonym Protein Names:La autoantigen; La ribonucleoprotein; Sjoegren syndrome type B antigen; SS-B
Protein Family:Lupus La protein
UniProt Gene Name:SSB  
UniProt Entry Name:LA_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells.
2.Incubate the cells for overnight at 37 °C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies Anti-SSB (Phospho-Ser366) Antibody, Anti-SSB Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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