SPA-Cys Recombinant Protein (RPPB4791)
- SKU:
- RPPB4791
- Product type:
- Recombinant Protein
- Size:
- 100mg
- Target:
- SPA-Cys
- Synonyms:
- Immunoglobulin G-binding protein A
- IgG-binding protein A
- Staphylococcal protein A
- SPA
- Source:
- Escherichia Coli
- Uniprot:
- P38507
Description
| Product Name: | SPA-Cys Recombinant Protein |
| Product Code: | RPPB4791 |
| Size: | 100mg |
| Target: | SPA-Cys |
| Synonyms: | Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | SPA protein was lyophilized with no additives. |
| Solubility: | It is recommended to reconstitute the lyophilized SPA in sterile 18M?-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by: (a) Analysis by HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC |
| UniProt Code: | P38507 |
Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The recombinant Protein A is genetically engineered protein and holds 5 IgG-binding regions of protein A. Recombinant Protein A functions basically the same as native Protein A and is perfect for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to human IgG1, IgG2 and IgG4, mouse IgG2a, IgG2b and IgG3 and rat IgG2c. Protein A also binds to total IgG from rabbit, pig, dog, cat, and guinea pig.
SPA-Cys Recombinant produced in E.Coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 297 amino acids and having a molecular mass of 33.5kDa containing little or no carbohydrate. Cell wall binding region, cell membrane binding region and albumin binding region were removed to ensure the highest specific IgG binding.
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