Smad1 (Phospho-Ser465) Transcription Factor Activity Assay
- SKU:
- TFAB00063
- Product Type:
- ELISA Kit
- ELISA Type:
- Transcription Factor Assay
- Analytes:
- Smad1 (Phospho-Ser465)
- Reactivity:
- Human
- Mouse
- Rat
Description
Smad1 (Phospho-Ser465)Transcription Factor Activity Assay
Based on the information found at www.assaygenie.com/smad1-phospho-ser465-transcription-factor-activity-assay/, the SMAD1 Phospho-Ser465 Transcription Factor Activity Assay kit is a cutting-edge tool designed for the accurate detection of SMAD1 phosphorylation at serine 465 and its role in transcription factor activity. This assay kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SMAD1 is a key player in the TGF-β signaling pathway, playing a critical role in regulating cell growth, differentiation, and apoptosis. Phosphorylation of SMAD1 at serine 465 is known to impact its transcription factor activity, influencing gene expression and cellular responses.
Understanding the regulation of SMAD1 phosphorylation is essential for unraveling the complexities of cell signaling pathways and disease mechanisms.With this SMAD1 Phospho-Ser465 Transcription Factor Activity Assay kit, researchers can delve deeper into the molecular mechanisms governing SMAD1 phosphorylation and its functional implications. By providing accurate measurements of SMAD1 phosphorylation status, this assay kit is an invaluable tool for advancing our understanding of signaling pathways and developing targeted therapies for various diseases.
| Product Name: | Smad1 (Phospho-Ser465) Transcription Factor Activity Assay |
| Product Code: | TFAB00063 |
| Target: | Smad1 (Phospho-Ser465) |
| Synonyms: | JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie Smad1 (Phospho-Ser465) transcription factor activity assay allows for the detection and qualitative analysis of Smad1 (Phospho-Ser465) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | SMAD1: transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 4q31 Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; nuclear inner membrane; intracellular; cytosol; nucleus Molecular Function:identical protein binding; protein binding; metal ion binding; receptor signaling protein activity; protein kinase binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity Biological Process: wound healing; cardiac muscle cell proliferation; primary microRNA processing; embryonic pattern specification; signal transduction; protein amino acid phosphorylation; mesodermal cell fate commitment; BMP signaling pathway; negative regulation of cell proliferation; homeostatic process; ureteric bud development; transforming growth factor beta receptor signaling pathway; midbrain development; inflammatory response; hindbrain development; response to drug; transcription, DNA-dependent; MAPKKK cascade; positive regulation of dendrite morphogenesis; response to organic nitrogen; SMAD protein complex assembly; positive regulation of osteoblast differentiation; gamete generation; cartilage development; positive regulation of transcription from RNA polymerase II promoter; osteoblast fate commitment |
| NCBI Summary: | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15797 |
| NCBI GenInfo Identifier: | 13633915 |
| NCBI Gene ID: | 4086 |
| NCBI Accession: | Q15797.1 |
| UniProt Secondary Accession: | Q15797,Q16636, Q9UFT8, A8KAJ0, D3DNZ9, |
| UniProt Related Accession: | Q15797 |
| Molecular Weight: | 465 |
| NCBI Full Name: | Mothers against decapentaplegic homolog 1 |
| NCBI Synonym Full Names: | SMAD family member 1 |
| NCBI Official Symbol: | SMAD1 |
| NCBI Official Synonym Symbols: | BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1 |
| NCBI Protein Information: | mothers against decapentaplegic homolog 1; MAD homolog 1; Mad-related protein 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; MAD, mothers against decapentaplegic homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1 |
| UniProt Protein Name: | Mothers against decapentaplegic homolog 1 |
| UniProt Synonym Protein Names: | JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1 |
| Protein Family: | Mothers against decapentaplegic |
| UniProt Gene Name: | SMAD1 |
| UniProt Entry Name: | SMAD1_HUMAN |
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