SEAP Reporter Gene Assay Kit (CV0010)

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SKU:

CV0010

Product Type:

Assay

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Description

SEAP Reporter Gene Assay Kit

Secreted Alkaline Phosphatase (SEAP) Reporter Gene is a really easy, sensitive and fast assay that utilizes enzyme activity of alkaline phosphatase to dephosphorylate the chemiluminescent alkaline phosphatase substrate CSPD into an unstable dioxetane anion which decomposes and emits light.

SEAP encodes a truncated form of the placental enzyme that lacks the membrane anchoring domain, thereby allowing the protein to secrete efficiently from transfected cells.

Changes in levels of SEAP activity detected in the culture medium are directly proportional to changes in intracellular concentrations of SEAP mRNA and protein.

Overview	Description
Size:	288 assays
Application:	Measurement the levels of SEAP in the culture medium of transfected cells.
Advantages & Features:	Convenient: a single set of cells is used for both the SEAP assay and another purpose. Time-saving protocol: results in 55 minutes due the elimination of cell lysate preparation. Cost-effective: allows performance directly in a microtiter plate. Sensitive: assayed even in low cell concentrations. Secreted from transfected cells into the culture medium.
Kit Contents:	3 x 96 W Solid Plate (white) 3 units of lid 50 μL Alkaline Phosphatase Standard 15 mL SEAP Substrate (Luminescence)
Shipping & Storage:	Shipping: Ambient Temperature. Storage: 4 ºC, except Component solution containing CSPD® Substrate (2-6 °C), Placental Alkaline Phosphatase Positive Control (-20 °C). Shelf Life: All components are stable for 2 years of receipt, if stored properly. Recommendations: Protect SEAP Substrate from light.
Safety:	This product is developed, designed and sold *exclusively for Research purposes and in vitro* use only (RUO)**. The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.