Anti-RuBisCO activase 항체의 (PACO24900)

Antibody Name:	RuBisCO activase Antibody (PACO24900)
Antibody SKU:	PACO24900
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:	Spinach
Immunogen:	Ribulose-1,5-bisphosphate carboxylase oxygenase (Native Protein)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western blot
All lanes: Ribulose-1,5-bisphosphate carboxylase oxygenase antibody at 5µg/ml
Lane 1: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.01µg/ml
Lane 2: Ribulose-1,5-bisphosphate carboxylase oxygenase at 0.1µg/ml
Lane 3: Ribulose-1,5-bisphosphate carboxylase oxygenase at 1µg/ml
Lane 4: Ribulose-1,5-bisphosphate carboxylase oxygenase at 10µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 55, 14 kDa
Observed band size: 55, 14 kDa
.

Background:

Ribulose-1,5-bisphosphate carboxylase oxygenase, most commonly known by the shorter name RuBisCO,is an enzyme involved in the Calvin cycle that catalyzes the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as glucose. RuBisCO catalyzes either the carboxylation or the oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen. RuBisCO is very important in terms of biological impact because it catalyzes the primary chemical reaction by which inorganic carbon permanently enters the biosphere. Many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle or the reverse Krebs cycle, but they make up a relatively minor portion of global net primary production. Phosphoenolpyruvate carboxylase PEPC only temporarily fixes carbon. RuBisCO is also the most abundant protein in leaves, and is considered to be the most abundant protein on Earth.It accounts for 50% of soluble leaf protein in C3 plants (20-30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5-9% of total leaf nitrogen).Given its important role in the biosphere, there are currently efforts to genetically engineer crop plants so as to contain more efficient RuBisCO.

Synonyms:

Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic (RA) (RuBisCO activase)

UniProt Protein Function:	Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P10871
NCBI GenInfo Identifier:	12643998
NCBI Gene ID:
NCBI Accession:	P10871.2
UniProt Secondary Accession:	P10871,Q43327
UniProt Related Accession:	P10871
Molecular Weight:	61.78 kDa
NCBI Full Name:	Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
NCBI Synonym Full Names:
NCBI Official Symbol:
NCBI Official Synonym Symbols:
NCBI Protein Information:
UniProt Protein Name:	Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
UniProt Synonym Protein Names:
Protein Family:	Ribulose bisphosphate carboxylase/oxygenase activase
UniProt Gene Name:	RA
UniProt Entry Name:

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)