Description

Product Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Key Information	Description
Product Name	Recombinant 2019-nCoV Nucleocapsid Protein, Biotinylated (His Tag)
Size	20µg
SKU	RPES0057
Synonyms	NP;2019-nCoV coronavirus NP Protein; 2019-nCoV np Protein; 2019-nCoV novel coronavirus Nucleoprotein Protein
Species	Virus
Expression host	Baculovirus-Insect Cells
Sequence	(Met1-Ala419(335Gly/Ala))
Accession	YP_009724397.2
Molar Mass	47.08 kDa
Tag	C-His
Purity	> 85 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per µg as determined by the LAL method.
Storage	Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, 500 mM NaCl, pH 7.0.
Reconstitution	Please refer to the printed manual for detailed information.