The Rat VWF (Von Willebrand Factor) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of VWF in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Von Willebrand Factor is a key player in the blood clotting process, playing a crucial role in hemostasis and thrombosis.
Dysregulation of VWF levels can lead to serious health consequences, making it a valuable biomarker for studying conditions such as von Willebrand disease and other clotting disorders.With the Rat VWF ELISA Kit, researchers can confidently investigate the role of VWF in various physiological and pathological processes, paving the way for potential therapeutic interventions and advancements in the field of hemostasis research.
Product Name:
Rat VWF (Von Willebrand Factor) ELISA Kit
SKU:
RTES00889
Target:
Rat VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VWF. You can calculate the concentration of Rat VWF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
95-111
94-107
Average (%)
94
103
101
1:4
Range (%)
91-106
81-92
83-96
Average (%)
97
86
90
1:8
Range (%)
88-104
83-96
82-94
Average (%)
96
88
89
1:16
Range (%)
88-101
84-94
87-100
Average (%)
95
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.56
3.62
0.43
1.57
3.87
Standard deviation
0.03
0.07
0.12
0.03
0.09
0.19
C V (%)
6.38
4.49
3.31
6.98
5.73
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat VWF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VWF in samples. No significant cross-reactivity or interference between Rat VWF and analogues was observed.
