Rat VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit
The Rat VEGF-A (Vascular Endothelial Cell Growth Factor-A) ELISA Kit is specifically designed for the accurate measurement of VEGF-A levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.VEGF-A is a key protein that plays a critical role in promoting vascular endothelial cell growth and angiogenesis. It is involved in various physiological and pathological processes, including wound healing, tumor growth, and ischemic diseases.
Studying VEGF-A levels can provide valuable insights into these biological processes and aid in the development of novel therapeutic interventions.With its reliable performance and ease of use, the Rat VEGF-A ELISA Kit is an essential tool for researchers studying angiogenesis, vascular biology, and related fields. Get accurate and reliable data with this advanced ELISA kit from Assay Genie.
Product Name:
Rat VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit
SKU:
RTES01205
Target:
Rat VEGF-A (Vascular Endothelial Cell Growth Factor A )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VEGF-A. You can calculate the concentration of Rat VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
86-98
96-112
Average (%)
95
93
103
1:4
Range (%)
88-99
84-99
87-102
Average (%)
94
91
94
1:8
Range (%)
94-107
83-96
85-99
Average (%)
100
88
91
1:16
Range (%)
89-103
80-90
87-101
Average (%)
95
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.9
170.39
948.99
89.62
158.2
968.59
Standard deviation
5.02
9.25
46.6
5.65
7.26
37.97
C V (%)
5.13
5.43
4.91
6.3
4.59
3.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat VEGF-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VEGF-A in samples. No significant cross-reactivity or interference between Rat VEGF-A and analogues was observed.
