Rat VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit
The Rat VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of VE-Cadherin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for research applications in the field of vascular biology.VE-Cadherin is a key player in maintaining vascular integrity and endothelial cell adhesion, crucial for proper blood vessel formation and function.
Dysregulation of VE-Cadherin has been implicated in various cardiovascular diseases, inflammatory conditions, and angiogenesis-related disorders, underscoring its importance as a biomarker in disease research and drug development.Overall, the Rat VE-Cadherin CLIA Kit offers researchers a valuable tool for studying endothelial cell biology, vascular function, and disease mechanisms, providing insights that could lead to new therapeutic interventions and improved patient outcomes.
Product Name:
Rat VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit
SKU:
RTES00075
Target:
Rat VE-Cadherin (Vascular Endothelial Cadherin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat VE-Cadherin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat VE-Cadherin. You can calculate the concentration of Rat VE-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
95-107
91-104
Average (%)
92
100
98
1:4
Range (%)
84-98
86-101
86-99
Average (%)
91
92
91
1:8
Range (%)
99-114
95-111
87-101
Average (%)
106
102
93
1:16
Range (%)
89-102
95-109
101-112
Average (%)
94
102
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
93-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.81
285.24
696.7
97.29
276.27
706.7
Standard deviation
8.18
30.12
46.96
8.75
29.37
64.17
C V (%)
8.72
10.56
6.74
8.99
10.63
9.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat VE-Cadherin concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VE-Cadherin in samples. No significant cross-reactivity or interference between Rat VE-Cadherin and analogues was observed.
