Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Product Name:
Rat UCHL3/UCH-L3 Recombinant Protein (RPES1035)
Product Code:
RPES1035
Size:
20µg
Species:
Rat
Expressed Host:
E. coli
Synonyms:
rCG_37146,RGD1561196
Accession:
Q91Y78
Sequence:
Glu 2-Ala 230
Fusion tag:
N-His
Activity:
Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/µg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
27.5 kDa
AP Mol Mass:
28 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
