The Rat TTR (Transthyretin) CLIA Kit is specifically designed for the precise measurement of transthyretin levels in rat serum, plasma, and tissue samples. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Transthyretin is a key protein involved in transporting thyroid hormones and retinol-binding protein, playing a critical role in maintaining proper neurological function and overall health. Dysregulation of transthyretin levels has been linked to various diseases, including familial amyloidotic polyneuropathy (FAP) and senile systemic amyloidosis, making it a valuable biomarker for studying these conditions and potentially developing new treatments.
Overall, the Rat TTR (Transthyretin) CLIA Kit is an essential tool for researchers looking to investigate the role of transthyretin in health and disease in rat models. Its high performance and reliability make it a valuable asset for advancing scientific knowledge and therapeutic development.
Product Name:
Rat TTR (Transthyretin) CLIA Kit
SKU:
RTES00571
Target:
Rat TTR (Transthyretin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TTR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TTR. You can calculate the concentration of Rat TTR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
87-101
96-112
Average (%)
90
92
102
1:4
Range (%)
84-99
93-105
92-104
Average (%)
91
98
97
1:8
Range (%)
100-117
96-108
94-110
Average (%)
107
102
101
1:16
Range (%)
95-109
97-114
91-102
Average (%)
101
105
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.16
4.69
13.98
2.36
4.27
14.61
Standard deviation
0.24
0.44
1.27
0.24
0.37
1.52
C V (%)
11.11
9.38
9.08
10.17
8.67
10.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TTR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TTR in samples. No significant cross-reactivity or interference between Rat TTR and analogues was observed.
