The Rat TSP-1 (Thrombospondin-1) ELISA Kit is specifically designed for the accurate measurement of thrombospondin-1 levels in rat serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Thrombospondin-1 is a key glycoprotein involved in cell-to-cell and cell-to-matrix interactions, playing a critical role in processes such as cell adhesion, migration, and tissue remodeling.
Its dysregulation has been linked to various diseases, including cancer, cardiovascular disorders, and fibrotic conditions, highlighting its importance as a biomarker for disease progression and treatment development.Overall, the Rat TSP-1 (Thrombospondin-1) ELISA Kit provides researchers with a powerful tool to investigate the role of thrombospondin-1 in disease pathogenesis and therapeutic interventions, leading to advancements in biomedical research and clinical applications.
Product Name:
Rat TSP-1 (Thrombospondin 1) ELISA Kit
SKU:
RTES00805
Target:
Rat TSP-1 (Thrombospondin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TSP-1. You can calculate the concentration of Rat TSP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
91-107
92-109
Average (%)
96
98
99
1:4
Range (%)
94-107
84-98
83-98
Average (%)
99
90
90
1:8
Range (%)
94-108
85-101
87-102
Average (%)
100
92
94
1:16
Range (%)
90-103
81-93
86-100
Average (%)
96
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.56
4.83
0.57
1.63
5.07
Standard deviation
0.03
0.06
0.2
0.03
0.07
0.16
C V (%)
5.66
3.85
4.14
5.26
4.29
3.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TSP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSP-1 in samples. No significant cross-reactivity or interference between Rat TSP-1 and analogues was observed.
