The Rat TSH (Thyroid Stimulating Hormone) ELISA Kit is a highly reliable and sensitive kit designed for the accurate measurement of TSH levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it suitable for a variety of research applications.TSH is a key hormone in the regulation of the thyroid gland, playing a crucial role in controlling metabolism and energy levels in the body.
Imbalances in TSH levels can indicate thyroid dysfunction, making it a valuable biomarker for studying thyroid disorders and related conditions.By using the Rat TSH ELISA Kit, researchers can accurately quantify TSH levels in rat samples, allowing for a better understanding of thyroid function and the development of potential treatments for thyroid-related diseases.
Product Name:
Rat TSH (Thyroid Stimulating Hormone) ELISA Kit
SKU:
RTES00816
Target:
Rat TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TSH. You can calculate the concentration of Rat TSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
96-111
87-102
Average (%)
101
102
95
1:4
Range (%)
93-107
85-97
95-108
Average (%)
101
92
100
1:8
Range (%)
98-112
79-91
91-107
Average (%)
106
86
98
1:16
Range (%)
97-114
86-96
94-107
Average (%)
105
91
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.2
7.9
38.1
4.6
7.5
38.1
Standard deviation
0.3
0.3
1.3
0.3
0.4
1.6
C V (%)
7.14
3.8
3.41
6.52
5.33
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TSH in samples. No significant cross-reactivity or interference between Rat TSH and analogues was observed.
