The Rat TRX (Thioredoxin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of thioredoxin levels in rat samples. Thioredoxin is a key protein involved in redox signaling and regulation, serving as a critical antioxidant and modulator of cellular processes.This CLIA kit offers reliable and reproducible results, making it ideal for a wide range of research applications in areas such as oxidative stress, inflammation, and cellular homeostasis.
By measuring thioredoxin levels, researchers can gain valuable insights into the role of this protein in various disease states and potentially identify therapeutic targets.Overall, the Rat TRX (Thioredoxin) CLIA Kit is a valuable tool for studying the impact of thioredoxin on cellular function and disease pathogenesis in rat models.
Product Name:
Rat TRX (Thioredoxin) CLIA Kit
SKU:
RTES00543
Target:
Rat TRX (Thioredoxin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TRX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TRX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TRX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TRX. You can calculate the concentration of Rat TRX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-99
88-104
101-113
Average (%)
90
95
107
1:4
Range (%)
89-101
90-102
100-118
Average (%)
94
95
108
1:8
Range (%)
94-109
89-105
92-103
Average (%)
100
96
98
1:16
Range (%)
87-100
90-108
98-112
Average (%)
92
98
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
98-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.62
365.29
1503.68
203.27
334.3
1632.61
Standard deviation
21.92
40.8
172.77
24.31
37.74
147.91
C V (%)
10.26
11.17
11.49
11.96
11.29
9.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TRX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TRX in samples. No significant cross-reactivity or interference between Rat TRX and analogues was observed.
