TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptors with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, longterm potentiation and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat TrkB/NTRK2 Recombinant Protein (RPES0133)
Product Code:
RPES0133
Size:
20µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
NTRK2,Trkb
Accession:
Q63604-Isoform T1
Sequence:
Met1-His429
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA.Immobilized rat NTRK2-His at 10 µg/ml (100 ?l/well) can bind biotinylated mouse BDNF , The EC50 of biotinylated mouse BDNF is 12.2-28.6 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat NTRK2 (Q63604-Isoform T1) (Met1-His429) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by SDS-PAGE
Mol Mass:
47.1 kDa
AP Mol Mass:
67 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
