The Rat TN-T (Troponin T) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Troponin T levels in rat serum, plasma, and tissue culture supernatants. This kit provides reliable and reproducible results, making it ideal for research applications in cardiology, toxicology, and drug development.Troponin T is a key protein involved in regulating muscle contraction, particularly in cardiac muscle. Elevated levels of Troponin T in the bloodstream are indicative of cardiac muscle damage, making it a valuable biomarker for diagnosing and monitoring heart-related conditions such as myocardial infarction and heart failure.
By utilizing the Rat TN-T CLIA Kit, researchers can effectively study the impact of various treatments and interventions on Troponin T levels, leading to a better understanding of cardiac function and the development of potential therapeutic strategies for heart disease.
Product Name:
Rat Tn-T (Troponin T) CLIA Kit
SKU:
RTES00033
Target:
Rat Tn-T (Troponin T)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TnTf/TNNT3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TnTf/TNNT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TnTf/TNNT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TnTf/TNNT3. You can calculate the concentration of Rat TnTf/TNNT3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
100-116
87-102
Average (%)
106
106
93
1:4
Range (%)
98-111
92-105
99-112
Average (%)
103
98
105
1:8
Range (%)
85-96
91-106
91-103
Average (%)
91
98
97
1:16
Range (%)
96-112
95-110
88-100
Average (%)
103
102
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
97-109
103
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.0
71.15
231.38
22.39
65.33
212.14
Standard deviation
2.12
7.46
18.7
2.21
5.64
21.87
C V (%)
8.83
10.48
8.08
9.87
8.63
10.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TnTf/TNNT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TnTf/TNNT3 in samples. No significant cross-reactivity or interference between Rat TnTf/TNNT3 and analogues was observed.
