The Rat TM (Thrombomodulin) ELISA Kit is specifically designed for the precise measurement of thrombomodulin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring reliable and consistent results for various research applications.Thrombomodulin is a vital protein known for its role in regulating blood coagulation and inflammation. It also plays a key part in maintaining vascular health and preventing thrombosis. Dysregulation of thrombomodulin levels has been linked to various diseases, including sepsis, cardiovascular disorders, and coagulopathies, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Overall, the Rat TM (Thrombomodulin) ELISA Kit provides researchers with a powerful tool for investigating the role of thrombomodulin in health and disease, ultimately contributing to advancements in the field of vascular biology and related disciplines.
Product Name:
Rat TM (Thrombomodulin) ELISA Kit
SKU:
RTES00801
Target:
Rat TM (Thrombomodulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
56.25 pg/mL
Detection range:
93.75-6000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TM. You can calculate the concentration of Rat TM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
92-105
89-104
Average (%)
104
98
97
1:4
Range (%)
89-103
80-91
83-95
Average (%)
94
86
88
1:8
Range (%)
91-104
79-93
82-95
Average (%)
97
85
89
1:16
Range (%)
90-102
79-90
86-98
Average (%)
96
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
283.86
553.59
2695.95
274.21
607.48
2867.75
Standard deviation
19.36
27.35
98.13
15.68
24.79
147.69
C V (%)
6.82
4.94
3.64
5.72
4.08
5.15
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TM in samples. No significant cross-reactivity or interference between Rat TM and analogues was observed.
