The Rat TLR2 (Toll-like Receptor 2) ELISA Kit is a reliable tool for the precise measurement of TLR2 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.TLR2 is a key receptor involved in the recognition of pathogens and activation of immune responses. It plays a critical role in the body's defense against infections and inflammatory processes.
By studying TLR2 levels, researchers can gain valuable insights into the immune system's functioning and its role in various diseases.With its high-quality components and user-friendly protocol, the Rat TLR2 ELISA Kit is a valuable resource for researchers investigating immune responses, infectious diseases, and inflammatory disorders in rat models. Trust this kit for reliable and insightful data that can contribute to advancing scientific knowledge and potential therapeutic interventions.
Product Name:
Rat TLR2 (Toll-Like Receptor 2) ELISA Kit
SKU:
RTES00753
Target:
Rat TLR2 (Toll-Like Receptor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TLR-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TLR-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TLR-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TLR-2. You can calculate the concentration of Rat TLR-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
86-102
85-95
Average (%)
106
93
90
1:4
Range (%)
92-108
84-96
83-96
Average (%)
99
90
90
1:8
Range (%)
89-102
85-96
85-99
Average (%)
96
90
92
1:16
Range (%)
95-106
82-94
85-97
Average (%)
100
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
2.1
7.93
1.01
2.01
8.17
Standard deviation
0.06
0.1
0.41
0.05
0.09
0.28
C V (%)
6.52
4.76
5.17
4.95
4.48
3.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TLR-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TLR-2 in samples. No significant cross-reactivity or interference between Rat TLR-2 and analogues was observed.
