Rat TIMP1 Recombinant Protein (RPPB2323)
- SKU:
- RPPB2323
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Rat
- Target:
- TIMP1
- Synonyms:
- Metalloproteinase inhibitor 1
- Tissue inhibitor of metalloproteinases 1
- TIMP-1
- TIMP1
- Source:
- Sf9 Insect cells
- Uniprot:
- P30120
Description
| Product Name: | Rat TIMP1 Recombinant Protein |
| Product Code: | RPPB2323 |
| Size: | 10µg |
| Species: | Rat |
| Target: | TIMP1 |
| Synonyms: | Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases 1, TIMP-1, TIMP1. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | TIMP1 Ligand protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | CSCAPTHPQT AFCNSDLVIR AKFMGSPEII ETTLYQRYEI KMTKMLKGFD AVGNATGFRF AYTPAMESLC GYVHKSQNRS EEFLIAGRLR NGNLHITACS FLVPWHNLSP AQQKAFVKTY SAGCGVCTVF PCSAIPCKLE SDSHCLWTDQ ILMGSEKGYQ SDHFACLPRN PDLCTWQYLG VSMTRSLPLA KAEAHHHHHH |
TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells.The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds.TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones.Increased TIMP1 levels are connected with squamous cell laryngeal carcinoma. TIMP1 overexpression is linked to gastric cancer.
TIMP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (24-217 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 200 amino acids and having a molecular mass of 22.3kDa.TIMP1 Ligand shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity. |
| NCBI Summary: | acts as an inhibitor of metalloprotease activity; may play a role in vascular tissue remodeling [RGD, Feb 2006] |
| UniProt Code: | P30120 |
| NCBI GenInfo Identifier: | 1174697 |
| NCBI Gene ID: | 116510 |
| NCBI Accession: | P30120.2 |
| UniProt Secondary Accession: | P30120,P70533, Q53YM7, |
| UniProt Related Accession: | P30120 |
| Molecular Weight: | 23,794 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 1 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 1 |
| NCBI Official Symbol: | Timp1 |
| NCBI Official Synonym Symbols: | Timp; TIMP-1 |
| NCBI Protein Information: | metalloproteinase inhibitor 1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1 |
| UniProt Protein Name: | Metalloproteinase inhibitor 1 |
| UniProt Synonym Protein Names: | Tissue inhibitor of metalloproteinases 1; TIMP-1 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | Timp1 |
| UniProt Entry Name: | TIMP1_RAT |
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