Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
The Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase-3) CLIA Kit is a highly sensitive assay designed for the precise measurement of TIMP-3 levels in rat tissues. This kit offers exceptional accuracy and reliability, allowing researchers to obtain consistent and trustworthy data for their studies.TIMP-3 is a vital protein that regulates the activity of metalloproteinases, enzymes involved in tissue remodeling and matrix degradation. Dysregulation of TIMP-3 has been implicated in various pathological conditions, including cancer, inflammation, and cardiovascular diseases.
By measuring TIMP-3 levels, researchers can gain insight into the mechanisms underlying these diseases and identify potential therapeutic targets.Whether investigating the role of TIMP-3 in disease progression or evaluating the efficacy of therapeutic interventions, the Rat TIMP-3 CLIA Kit provides researchers with a valuable tool for advancing their research goals. Trust in the accuracy and precision of this kit to deliver reliable results for your studies.
Product Name:
Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) CLIA Kit
SKU:
RTES00558
Target:
Rat TIMP-3 (Tissue Inhibitors of Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TIMP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TIMP-3. You can calculate the concentration of Rat TIMP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-103
85-96
Average (%)
93
97
91
1:4
Range (%)
99-113
84-98
91-104
Average (%)
106
90
98
1:8
Range (%)
93-104
87-99
86-97
Average (%)
99
94
91
1:16
Range (%)
89-102
92-106
91-105
Average (%)
94
99
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
100
EDTA plasma (n=5)
100-113
107
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.7
172.7
845.69
108.13
174.75
899.24
Standard deviation
12.54
13.59
64.53
12.33
15.45
65.28
C V (%)
12.09
7.87
7.63
11.4
8.84
7.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TIMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TIMP-3 in samples. No significant cross-reactivity or interference between Rat TIMP-3 and analogues was observed.
