The Rat THP (Tamm-Horsfall Glycoprotein) CLIA Kit is specifically designed for the accurate quantification of THP levels in rat serum, plasma, and urine samples. This kit provides high sensitivity and specificity, allowing for reliable and reproducible results in various research applications.THP, also known as uromodulin, is a glycoprotein found in the kidneys and plays a critical role in renal function and urinary tract health. Abnormal levels of THP have been associated with kidney diseases, urinary tract infections, and other renal disorders, making it a valuable biomarker for studying these conditions.
With this Rat THP CLIA Kit, researchers can easily measure THP levels in rat samples, aiding in the diagnosis, monitoring, and understanding of kidney and urinary tract-related diseases. Get accurate and dependable results with this comprehensive kit from Assay Genie.
Product Name:
Rat THP (Tamm–Horsfall Glycoprotein) CLIA Kit
SKU:
RTES00535
Target:
Rat THP (Tamm–Horsfall Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat THP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat THP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat THP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat THP. You can calculate the concentration of Rat THP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
86-96
99-111
Average (%)
109
91
105
1:4
Range (%)
91-103
92-103
90-103
Average (%)
97
97
95
1:8
Range (%)
99-114
91-103
87-101
Average (%)
107
97
93
1:16
Range (%)
87-102
88-102
102-116
Average (%)
95
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
4.34
19.58
2.02
4.71
20.0
Standard deviation
0.2
0.34
1.53
0.24
0.54
1.51
C V (%)
10.47
7.83
7.81
11.88
11.46
7.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat THP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat THP in samples. No significant cross-reactivity or interference between Rat THP and analogues was observed.
