The Rat TH (Tyrosine Hydroxylase) ELISA Kit is specifically designed for the quantitative measurement of tyrosine hydroxylase levels in rat serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Tyrosine hydroxylase is a key enzyme involved in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine. Dysregulation of tyrosine hydroxylase activity has been implicated in various neurological disorders, such as Parkinson's disease and schizophrenia.
Therefore, the Rat TH ELISA Kit is an essential tool for studying the role of tyrosine hydroxylase in these conditions and exploring potential therapeutic interventions.With its easy-to-use protocol and robust performance, the Rat TH ELISA Kit is a valuable asset for researchers studying the intricate mechanisms of neurotransmitter synthesis and neuronal function in rat models. Trust Assay Genie for high-quality ELISA kits that deliver accurate and meaningful results for your research needs. Visit the website for more details: www.assaygenie.com/rat-th-tyrosine-hydroxylase-elisa-kit-rtes01007/
Product Name:
Rat TH (Tyrosine Hydroxylase) ELISA Kit
SKU:
RTES01007
Target:
Rat TH (Tyrosine Hydroxylase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TH. You can calculate the concentration of Rat TH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
85-98
93-106
Average (%)
97
90
98
1:4
Range (%)
93-106
83-97
83-98
Average (%)
100
89
90
1:8
Range (%)
91-105
80-90
86-99
Average (%)
97
85
93
1:16
Range (%)
92-103
80-94
88-103
Average (%)
97
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
84-99
90
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.14
4.77
0.5
1.04
5.2
Standard deviation
0.03
0.06
0.19
0.03
0.06
0.17
C V (%)
5.77
5.26
3.98
6.0
5.77
3.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat TH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TH in samples. No significant cross-reactivity or interference between Rat TH and analogues was observed.
