Rat TGF- beta3 (Transforming Growth Factor Beta 3) CLIA Kit
The Rat TGF-Beta3 (Transforming Growth Factor Beta 3) CLIA Kit is a powerful tool designed for the precise and reliable measurement of TGF-Beta3 levels in rat samples such as serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, allowing researchers to obtain accurate and reproducible results for a variety of experimental applications.TGF-Beta3 is a key signaling molecule involved in cell growth, differentiation, and immune regulation. It plays a critical role in many biological processes, including tissue regeneration, wound healing, and immune response modulation.
Dysregulation of TGF-Beta3 signaling has been implicated in various diseases, such as cancer, fibrosis, and autoimmune disorders.By utilizing the Rat TGF-Beta3 CLIA Kit, researchers can gain valuable insights into the role of TGF-Beta3 in health and disease. This kit enables the quantification of TGF-Beta3 levels in rat samples with precision and accuracy, making it an indispensable tool for advancing our understanding of TGF-Beta3 signaling pathways and developing potential therapeutic interventions.
Product Name:
Rat TGF- beta3 (Transforming Growth Factor Beta 3) CLIA Kit
SKU:
RTES00569
Target:
Rat TGF- beta3 (Transforming Growth Factor Beta 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-β3. You can calculate the concentration of Rat TGF-β3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
103-115
86-100
Average (%)
108
109
92
1:4
Range (%)
88-99
100-116
96-109
Average (%)
93
107
103
1:8
Range (%)
88-99
86-99
97-108
Average (%)
94
93
102
1:16
Range (%)
86-98
102-120
88-103
Average (%)
92
110
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
98-113
106
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.47
67.02
189.29
28.93
63.82
170.93
Standard deviation
2.95
6.23
21.24
3.45
6.26
10.63
C V (%)
10.74
9.3
11.22
11.93
9.81
6.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-β3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TGF-β3 in samples. No significant cross-reactivity or interference between Rat TGF-β3 and analogues was observed.
