The Rat SYP (Synaptophysin) ELISA Kit is specifically designed for the precise measurement of synaptophysin levels in rat serum, plasma, and tissue lysate samples. This kit offers exceptional sensitivity and specificity, guaranteeing highly accurate and consistent results, making it perfect for various research purposes.Synaptophysin is a key protein found in synaptic vesicles and is essential for neurotransmitter release and synaptic plasticity.
It plays a crucial role in processes such as learning and memory, making it a valuable biomarker for studying neuronal function and synaptic transmission in various neurological disorders.With its reliable performance and broad applications, the Rat SYP (Synaptophysin) ELISA Kit is a valuable tool for researchers studying neurobiology, neuroscience, and synaptic function in preclinical and clinical studies.
Product Name:
Rat SYP (Synaptophysin) ELISA Kit
SKU:
RTES00773
Target:
Rat SYP (Synaptophysin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SYP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SYP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SYP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SYP. You can calculate the concentration of Rat SYP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
93-105
97-114
Average (%)
92
99
104
1:4
Range (%)
89-102
81-90
86-97
Average (%)
94
85
92
1:8
Range (%)
87-100
79-91
86-99
Average (%)
93
85
92
1:16
Range (%)
87-99
81-94
88-103
Average (%)
92
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.88
5.79
16.42
2.06
6.19
15.32
Standard deviation
0.1
0.28
0.66
0.14
0.33
0.74
C V (%)
5.32
4.84
4.02
6.8
5.33
4.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SYP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
