Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
The Rat SPD (Surfactant Associated Protein D) ELISA Kit is specifically designed for the precise quantification of pulmonary surfactant associated protein D levels in rat serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Pulmonary surfactant associated protein D is a critical component involved in respiratory function, particularly in the maintenance of lung surfactant levels and pulmonary defense mechanisms.
Dysregulation of SPD has been associated with various respiratory conditions such as acute respiratory distress syndrome (ARDS) and interstitial lung diseases, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.With this ELISA kit, researchers can confidently measure SPD levels in rat samples, aiding in the understanding of respiratory disorders and the development of targeted treatments.
Product Name:
Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
SKU:
RTES00697
Target:
Rat SPD (Pulmonary Surfactant Associated Protein D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SP-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SP-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SP-D. You can calculate the concentration of Rat SP-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
90-105
91-103
Average (%)
101
96
97
1:4
Range (%)
91-103
79-89
86-98
Average (%)
97
85
92
1:8
Range (%)
92-104
82-95
82-95
Average (%)
98
87
87
1:16
Range (%)
87-102
82-91
81-94
Average (%)
94
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
252.07
590.57
2301.76
247.61
552.21
2378.08
Standard deviation
16.28
25.1
90.0
15.08
33.08
101.07
C V (%)
6.46
4.25
3.91
6.09
5.99
4.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SP-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SP-D in samples. No significant cross-reactivity or interference between Rat SP-D and analogues was observed.
