The Rat SIGA (Secretory Immunoglobulin A) ELISA Kit is specifically designed for the quantitative measurement of secretory immunoglobulin A levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Secretory immunoglobulin A (SIGA) is an important component of the immune system, playing a key role in protecting mucosal surfaces from pathogens and allergens.
Studies have shown that alterations in SIGA levels are associated with various inflammatory and infectious diseases, making it a valuable biomarker for immunological research and diagnostics.Overall, the Rat SIGA ELISA Kit provides researchers with a reliable tool for studying the role of secretory immunoglobulin A in immune responses and disease pathogenesis, ultimately aiding in the development of novel therapeutic strategies.
Product Name:
Rat sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
RTES00729
Target:
Rat sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sIgA. You can calculate the concentration of Rat sIgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
99-111
90-104
Average (%)
97
105
97
1:4
Range (%)
92-104
83-95
88-98
Average (%)
99
89
93
1:8
Range (%)
89-102
80-92
84-96
Average (%)
94
86
90
1:16
Range (%)
91-103
82-94
87-99
Average (%)
97
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.22
18.16
98.54
9.57
19.43
108.06
Standard deviation
0.56
0.89
4.69
0.53
0.86
3.44
C V (%)
6.07
4.9
4.76
5.54
4.43
3.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat sIgA in samples. No significant cross-reactivity or interference between Rat sIgA and analogues was observed.
