The Rat SELP (P-Selectin) CLIA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of P-Selectin levels in rat serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for a variety of research applications.P-Selectin is a cell adhesion molecule that plays a key role in inflammation and immune response by facilitating the interaction between leukocytes and endothelial cells. Dysregulation of P-Selectin has been linked to various inflammatory conditions, including cardiovascular diseases, arthritis, and sepsis.
Therefore, the Rat SELP CLIA Kit is essential for studying these diseases and developing potential therapeutic interventions.Overall, the Rat SELP (P-Selectin) CLIA Kit offers researchers a valuable tool for investigating the role of P-Selectin in various physiological and pathological processes, ultimately contributing to advancements in the field of inflammation and immune response research.
Product Name:
Rat SELP (P-Selectin) CLIA Kit
SKU:
RTES00490
Target:
Rat SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SELP. You can calculate the concentration of Rat SELP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
101-118
98-113
Average (%)
109
108
104
1:4
Range (%)
100-114
87-97
91-107
Average (%)
107
92
99
1:8
Range (%)
95-108
95-109
97-111
Average (%)
102
100
104
1:16
Range (%)
86-99
95-111
101-119
Average (%)
91
102
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.27
528.18
1716.48
219.92
502.6
1571.84
Standard deviation
17.63
37.45
130.97
18.36
58.2
145.24
C V (%)
8.23
7.09
7.63
8.35
11.58
9.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SELP in samples. No significant cross-reactivity or interference between Rat SELP and analogues was observed.
