The Rat Renin CLIA Kit is specifically designed for the reliable and accurate quantification of renin levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing researchers with trustworthy and reproducible results for a variety of experimental applications.Renin is a key enzyme involved in the regulation of blood pressure and fluid balance through the renin-angiotensin system.
Dysregulation of renin levels can lead to hypertension and various cardiovascular disorders, highlighting the importance of measuring renin levels in research studies. This kit serves as a valuable tool for studying the role of renin in health and disease, providing insights into potential therapeutic interventions for related conditions.
Product Name:
Rat REN (Renin) CLIA Kit
SKU:
RTES00023
Target:
Rat REN (Renin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat REN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat REN. You can calculate the concentration of Rat REN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
100-117
99-112
Average (%)
102
108
106
1:4
Range (%)
102-116
87-99
90-106
Average (%)
109
92
97
1:8
Range (%)
94-106
91-103
88-102
Average (%)
100
96
94
1:16
Range (%)
86-97
99-115
96-111
Average (%)
91
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-110
103
EDTA plasma (n=5)
96-110
103
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.24
42.13
218.1
22.99
45.88
224.12
Standard deviation
1.99
3.3
23.93
2.4
4.81
18.15
C V (%)
8.21
7.83
10.97
10.44
10.48
8.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat REN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat REN in samples. No significant cross-reactivity or interference between Rat REN and analogues was observed.
