The Rat PTX3 (Pentraxin 3) ELISA Kit is a powerful tool for accurately measuring levels of PTX3 in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it suitable for various research purposes.PTX3, a member of the pentraxin family, is a key player in the immune response, inflammation, and tissue repair processes.
Its levels have been linked to various pathological conditions, including infectious diseases, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Rat PTX3 ELISA Kit is a reliable and efficient tool for researchers looking to explore the role of PTX3 in health and disease in rat models.
Product Name:
Rat PTX3 (Pentraxin 3) ELISA Kit
SKU:
RTES00836
Target:
Rat PTX3 (Pentraxin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PTX-3/TSG-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTX-3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTX-3/TSG-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PTX-3/TSG-14. You can calculate the concentration of Rat PTX-3/TSG-14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
96-112
94-104
Average (%)
101
104
99
1:4
Range (%)
93-109
83-94
83-93
Average (%)
100
88
88
1:8
Range (%)
88-100
84-100
85-97
Average (%)
94
91
92
1:16
Range (%)
89-102
84-97
88-102
Average (%)
96
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
90-102
97
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.27
3.74
0.5
1.27
3.93
Standard deviation
0.03
0.06
0.16
0.03
0.05
0.14
C V (%)
5.88
4.72
4.28
6.0
3.94
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PTX-3/TSG-14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTX-3/TSG-14 in samples. No significant cross-reactivity or interference between Rat PTX-3/TSG-14 and analogues was observed.
