The Rat PTN (Pleiotrophin) CLIA Kit is a highly reliable and sensitive assay designed for the precise measurement of pleiotrophin levels in rat samples. This kit is ideal for researchers studying the role of pleiotrophin in various biological processes, including cell growth, development, and disease progression.Pleiotrophin is a key protein that plays a critical role in cell growth, survival, and differentiation. It is involved in various cellular processes, making it an important biomarker for studying diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
With its high specificity and accuracy, the Rat PTN CLIA Kit offers researchers a powerful tool for conducting detailed studies on pleiotrophin biology and its implications in disease pathology. This kit provides reliable and reproducible results, making it a valuable asset in research laboratories.
Product Name:
Rat PTN (Pleiotrophin) CLIA Kit
SKU:
RTES00458
Target:
Rat PTN (Pleiotrophin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PTN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PTN. You can calculate the concentration of Rat PTN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
102-116
101-118
Average (%)
90
109
110
1:4
Range (%)
91-106
93-108
94-108
Average (%)
97
99
101
1:8
Range (%)
94-111
103-115
88-103
Average (%)
101
109
95
1:16
Range (%)
99-113
93-106
101-118
Average (%)
107
100
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.39
92.48
356.72
38.23
84.27
324.26
Standard deviation
3.52
9.58
35.03
4.04
6.68
34.5
C V (%)
9.17
10.36
9.82
10.57
7.93
10.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PTN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTN in samples. No significant cross-reactivity or interference between Rat PTN and analogues was observed.
