Rat PTHRP (Parathyroid Hormone Related Protein) CLIA Kit
The Rat PTHRP (Parathyroid Hormone-Related Protein) CLIA Kit is a powerful tool for the quantitative detection of PTHRP levels in rat samples. This kit offers high sensitivity and precision, ensuring accurate and consistent results for researchers studying PTHRP in the context of bone metabolism, cancer, and other physiological processes.PTHRP is a key regulator of calcium homeostasis and bone development, playing a crucial role in skeletal growth and remodeling. Dysregulation of PTHRP expression has been linked to various conditions such as osteoporosis, hypercalcemia, and certain types of cancer.
The Rat PTHRP CLIA Kit provides researchers with a reliable method for investigating the role of PTHRP in these diseases and potentially identifying new therapeutic targets.Overall, the Rat PTHRP CLIA Kit is a valuable tool for researchers seeking to better understand the functions of PTHRP and its implications in health and disease. Its high performance and ease of use make it an essential resource for advancing scientific knowledge in the field of endocrinology and related research areas.
Product Name:
Rat PTHRP (Parathyroid Hormone Related Protein) CLIA Kit
SKU:
RTES00428
Target:
Rat PTHRP (Parathyroid Hormone Related Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PTHrP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PTHrP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PTHrP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PTHrP. You can calculate the concentration of Rat PTHrP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
99-115
92-107
Average (%)
96
107
99
1:4
Range (%)
86-98
89-102
97-115
Average (%)
93
94
105
1:8
Range (%)
87-99
100-115
95-108
Average (%)
92
108
103
1:16
Range (%)
103-115
86-97
87-103
Average (%)
109
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
99-110
104
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.74
43.79
201.45
25.3
44.56
215.51
Standard deviation
3.53
3.1
22.3
2.35
3.2
22.15
C V (%)
12.73
7.08
11.07
9.29
7.18
10.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PTHrP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PTHrP in samples. No significant cross-reactivity or interference between Rat PTHrP and analogues was observed.
