The Rat PSA (Prostate Specific Antigen) ELISA Kit is specifically designed for the precise measurement of PSA levels in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and consistent results, making it an indispensable tool in various research studies.PSA is a vital biomarker for prostate cancer, playing a crucial role in the early detection and monitoring of this prevalent malignancy.
By measuring PSA levels, researchers can gain valuable insights into disease progression, treatment efficacy, and potential therapeutic interventions.By utilizing the Rat PSA ELISA Kit, researchers can advance their understanding of prostate cancer biology and potentially contribute to the development of innovative diagnostic and treatment strategies. Order now and take your research to the next level with this reliable and efficient ELISA kit.
Product Name:
Rat PSA (Prostate Specific Antigen) ELISA Kit
SKU:
RTES00663
Target:
Rat PSA (Prostate Specific Antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PSA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PSA. You can calculate the concentration of Rat PSA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
97-109
96-111
Average (%)
94
102
103
1:4
Range (%)
89-104
83-97
85-98
Average (%)
96
89
91
1:8
Range (%)
91-105
87-97
80-93
Average (%)
98
92
87
1:16
Range (%)
91-104
81-94
85-100
Average (%)
96
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
96-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
0.85
4.01
0.52
0.82
3.68
Standard deviation
0.03
0.05
0.18
0.03
0.04
0.14
C V (%)
5.77
5.88
4.49
5.77
4.88
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PSA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PSA in samples. No significant cross-reactivity or interference between Rat PSA and analogues was observed.
