The Rat POSTN/OSF-2 (Periostin) ELISA Kit is specifically designed for the accurate measurement of periostin levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Periostin, also known as osteoblast-specific factor 2 (OSF-2), is a crucial protein that plays a key role in tissue development and repair. It is involved in the regulation of cell adhesion, migration, and proliferation, making it a valuable biomarker for studying various physiological and pathological processes.
With the Rat POSTN/OSF-2 ELISA Kit, researchers can effectively investigate the role of periostin in conditions such as fibrosis, cancer, and cardiovascular diseases. Its reliable performance and easy-to-use format make it an essential tool for advancing research in these areas and potentially developing new therapeutic strategies.
Product Name:
Rat POSTN/OSF-2 (Periostin) ELISA Kit
SKU:
RTES01024
Target:
Rat POSTN/OSF-2 (Periostin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat POSTN/OSF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat POSTN/OSF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat POSTN/OSF-2. You can calculate the concentration of Rat POSTN/OSF-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
98-111
90-103
Average (%)
95
104
96
1:4
Range (%)
86-100
82-92
87-101
Average (%)
92
87
94
1:8
Range (%)
90-103
85-97
89-99
Average (%)
97
91
94
1:16
Range (%)
90-105
85-101
86-99
Average (%)
96
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
378.75
745.41
3208.87
401.0
812.43
2919.07
Standard deviation
22.38
44.35
133.17
23.58
41.27
149.75
C V (%)
5.91
5.95
4.15
5.88
5.08
5.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat POSTN/OSF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Rat POSTN/OSF-2 and analogues was observed.
