Rat PICP (Procollagen I C-terminal Propeptide) CLIA Kit
The Rat PICP (Procollagen I C-terminal propeptide) CLIA Kit is a reliable and accurate tool for quantifying levels of PICP in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.PICP is a key biomarker in the field of collagen research, as it serves as a marker for the synthesis of type I collagen, the most abundant form of collagen in the body.
Monitoring PICP levels can provide valuable insights into collagen metabolism and turnover, making it a valuable tool for studying conditions such as fibrosis, osteoporosis, and arthritis.With its ease of use and robust performance, the Rat PICP CLIA Kit is an indispensable tool for researchers seeking to better understand the role of collagen in health and disease.
Product Name:
Rat PICP (Procollagen I C-terminal Propeptide) CLIA Kit
SKU:
RTES00439
Target:
Rat PICP (Procollagen I C-terminal Propeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PICP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PICP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PICP. You can calculate the concentration of Rat PICP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
88-103
91-101
Average (%)
104
95
96
1:4
Range (%)
94-105
88-100
98-113
Average (%)
99
95
105
1:8
Range (%)
88-99
99-115
100-114
Average (%)
93
107
108
1:16
Range (%)
102-114
91-102
95-111
Average (%)
108
97
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
97
EDTA plasma (n=5)
97-113
104
Cell culture media (n=5)
99-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.82
9.74
0.96
2.54
9.67
Standard deviation
0.1
0.21
0.96
0.12
0.24
0.86
C V (%)
10.64
7.45
9.86
12.5
9.45
8.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PICP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PICP in samples. No significant cross-reactivity or interference between Rat PICP and analogues was observed.
