The Rat PI Proinsulin ELISA Kit is a highly sensitive and specific assay designed to accurately measure proinsulin levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.Proinsulin is a precursor to insulin and is crucial in regulating blood sugar levels. Changes in proinsulin levels can indicate insulin production and secretion dysfunction, making it a valuable biomarker in studying conditions such as diabetes and metabolic disorders.
With the Rat PI Proinsulin ELISA Kit, researchers can confidently analyze proinsulin levels in rats, enabling further understanding of the mechanisms underlying diabetes and other related conditions. Get accurate and reliable results with this essential tool for your research needs.
Product Name:
Rat PI (Proinsulin) ELISA Kit
SKU:
RTES00648
Target:
Rat PI (Proinsulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PI. You can calculate the concentration of Rat PI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
92-105
86-101
Average (%)
96
98
93
1:4
Range (%)
100-117
87-99
91-107
Average (%)
108
93
99
1:8
Range (%)
95-107
87-99
97-111
Average (%)
102
94
103
1:16
Range (%)
95-108
86-96
90-105
Average (%)
102
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
89-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.0
7.4
1.2
1.9
7.7
Standard deviation
0.1
0.1
0.3
0.1
0.1
0.3
C V (%)
9.09
5.0
4.05
8.33
5.26
3.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PI in samples. No significant cross-reactivity or interference between Rat PI and analogues was observed.
