Rat PDGFRL (Platelet Derived Growth Factor Receptor Like Protein) CLIA Kit
The Rat PDGFR-L (Platelet-Derived Growth Factor Receptor-Like Protein) CLIA Kit is a powerful tool for the quantitative measurement of PDGFR-L levels in rat samples. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in various types of biological samples.PDGFR-L is a key protein that plays a critical role in cell growth, differentiation, and survival. It is involved in various physiological processes, including tissue repair, development, and maintenance.
Dysregulation of PDGFR-L has been linked to a range of diseases, including cancer and fibrosis, highlighting its importance as a potential therapeutic target and biomarker.With the Rat PDGFR-L CLIA Kit, researchers can gain valuable insights into the role of PDGFR-L in disease pathogenesis and progression, ultimately leading to the development of targeted therapies and improved patient outcomes.
Product Name:
Rat PDGFRL (Platelet Derived Growth Factor Receptor Like Protein) CLIA Kit
SKU:
RTES00452
Target:
Rat PDGFRL (Platelet Derived Growth Factor Receptor Like Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGFRL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PDGFRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGFRL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PDGFRL. You can calculate the concentration of Rat PDGFRL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-97
91-103
98-112
Average (%)
90
97
105
1:4
Range (%)
94-109
87-103
96-113
Average (%)
101
94
104
1:8
Range (%)
93-107
87-101
93-107
Average (%)
101
93
100
1:16
Range (%)
92-104
92-108
103-116
Average (%)
98
99
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.25
138.33
420.63
51.15
126.82
397.57
Standard deviation
6.11
15.7
36.68
6.05
12.99
27.23
C V (%)
11.47
11.35
8.72
11.83
10.24
6.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PDGFRL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PDGFRL in samples. No significant cross-reactivity or interference between Rat PDGFRL and analogues was observed.
