Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA kit
The Rat PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) ELISA Kit is specifically designed for the accurate measurement of PCSK9 levels in rat serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for researchers in various fields.PCSK9 is a vital protein involved in the regulation of cholesterol levels in the body.
Dysregulation of PCSK9 has been linked to cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. By using the Rat PCSK9 ELISA Kit, researchers can gain valuable insights into the role of PCSK9 in disease progression and treatment strategies.
Product Name:
Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA kit
SKU:
RTES01104
Target:
Rat PCSK9 (Proprotein convertase subtilisin/kexin type 9) ELISA kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PCSK9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCSK9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCSK9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PCSK9. You can calculate the concentration of Rat PCSK9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
100-113
87-100
Average (%)
100
106
93
1:4
Range (%)
92-106
82-94
87-98
Average (%)
98
88
93
1:8
Range (%)
92-104
86-98
86-98
Average (%)
97
92
92
1:16
Range (%)
88-99
85-98
83-95
Average (%)
94
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.46
4.92
20.24
2.65
4.74
20.78
Standard deviation
0.16
0.26
1.0
0.16
0.2
0.78
C V (%)
6.5
5.28
4.94
6.04
4.22
3.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PCSK9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCSK9 in samples. No significant cross-reactivity or interference between Rat PCSK9 and analogues was observed.
