Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit
The Rat PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide) ELISA Kit is a powerful tool for measuring PACAP levels in rat samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results for a variety of research applications.PACAP is a neuropeptide that plays a critical role in neuroprotection, neurotransmission, and neurogenesis. It has been implicated in various physiological and pathological processes, including stress response, immune regulation, and neuronal development.
As a key player in the nervous system, PACAP serves as a valuable biomarker for investigating neurological disorders and potential therapeutic interventions.Get reliable data and valuable insights with the Rat PACAP ELISA Kit, a must-have for researchers studying the intricate functions of PACAP in health and disease.
Product Name:
Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit
SKU:
RTES01018
Target:
Rat PACAP (Pituitary Adenylate Cyclase Activating Polypeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PACAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PACAP. You can calculate the concentration of Rat PACAP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
100-111
87-101
Average (%)
102
105
94
1:4
Range (%)
89-105
84-96
87-100
Average (%)
97
90
93
1:8
Range (%)
91-104
83-95
87-98
Average (%)
97
90
92
1:16
Range (%)
88-101
85-99
89-100
Average (%)
93
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.36
71.27
183.41
29.28
75.79
175.78
Standard deviation
1.45
3.04
5.54
1.48
4.49
9.44
C V (%)
5.3
4.27
3.02
5.05
5.92
5.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat PACAP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PACAP in samples. No significant cross-reactivity or interference between Rat PACAP and analogues was observed.
