The Rat OSM (Oncostatin M) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of OSM levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for research applications in the fields of oncology, immunology, and inflammation.Oncostatin M is a cytokine that belongs to the interleukin-6 family, playing a crucial role in inflammation, immune response, and tissue regeneration.
It is involved in various physiological and pathological processes, making it a valuable biomarker for studying diseases such as cancer, inflammatory diseases, and autoimmune disorders.With its user-friendly protocol and high performance, the Rat OSM CLIA Kit offers researchers a powerful tool for studying the role of OSM in health and disease, paving the way for the development of potential therapeutic interventions.
Product Name:
Rat OSM (Oncostatin-M) CLIA Kit
SKU:
RTES00416
Target:
Rat OSM (Oncostatin-M)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat OSM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OSM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OSM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat OSM. You can calculate the concentration of Rat OSM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
89-104
96-112
Average (%)
91
95
104
1:4
Range (%)
99-114
87-99
102-118
Average (%)
105
94
110
1:8
Range (%)
97-111
103-115
86-98
Average (%)
103
108
91
1:16
Range (%)
98-115
85-97
97-113
Average (%)
105
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
101-113
107
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.33
44.39
198.79
29.12
43.77
215.88
Standard deviation
3.1
3.97
15.55
2.89
4.57
17.72
C V (%)
11.34
8.94
7.82
9.92
10.44
8.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat OSM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat OSM in samples. No significant cross-reactivity or interference between Rat OSM and analogues was observed.
