Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
The Rat NOS3 (eNOS) Nitric Oxide Synthase 3 Endothelial CLIA Kit is a reliable and accurate tool for detecting NOS3 levels in rat samples. This kit offers high sensitivity and specificity, allowing for precise and consistent results in serum, plasma, and cell culture supernatants.NOS3, also known as endothelial nitric oxide synthase, plays a vital role in nitric oxide production and vascular function. Dysregulation of NOS3 is associated with various diseases, including cardiovascular disorders and hypertension.
Studying NOS3 levels can provide valuable insights into the mechanisms underlying these conditions and aid in the development of therapeutic strategies.With its advanced technology and user-friendly protocol, the Rat NOS3 CLIA Kit is an essential tool for researchers investigating the role of NOS3 in physiological and pathological processes in rats. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit
SKU:
RTES00200
Target:
Rat NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3/eNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat NOS3/eNOS. You can calculate the concentration of Rat NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
101-118
94-110
Average (%)
91
109
101
1:4
Range (%)
94-109
94-109
101-116
Average (%)
100
101
109
1:8
Range (%)
101-115
99-113
91-104
Average (%)
108
107
97
1:16
Range (%)
91-107
87-102
100-114
Average (%)
98
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
92-105
99
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.14
61.4
193.55
21.99
66.12
195.86
Standard deviation
2.79
5.53
14.83
2.58
5.2
13.67
C V (%)
11.56
9.01
7.66
11.73
7.86
6.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat NOS3/eNOS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed.
