Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
The Rat NOS1 (nNOS) Nitric Oxide Synthase 1 Neuronal ELISA Kit is specifically designed for the precise detection of NOS1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.NOS1, or neuronal nitric oxide synthase 1, is an important enzyme responsible for the production of nitric oxide in neuronal cells. Nitric oxide plays a crucial role in various physiological processes, including neurotransmission, immune response, and vasodilation.
Dysregulation of NOS1 has been implicated in numerous diseases, such as neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.By accurately measuring NOS1 levels, researchers can gain valuable insights into the role of nitric oxide signaling in normal and pathological conditions, facilitating the development of novel therapeutic strategies. With its high performance and reliability, the Rat NOS1 Neuronal ELISA Kit is an indispensable tool for studying the complex mechanisms underlying nitric oxide-mediated signaling pathways.
Product Name:
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal) ELISA Kit
SKU:
RTES01008
Target:
Rat NOS1/nNOS (Nitric Oxide Synthase 1, Neuronal)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOS1/nNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NOS1/nNOS. You can calculate the concentration of Rat NOS1/nNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
98-113
88-101
Average (%)
98
104
94
1:4
Range (%)
96-112
84-96
91-105
Average (%)
104
91
97
1:8
Range (%)
101-113
87-101
94-111
Average (%)
107
92
102
1:16
Range (%)
96-108
82-95
96-109
Average (%)
102
88
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.6
4.5
0.5
1.4
4.7
Standard deviation
0.03
0.07
0.19
0.03
0.08
0.15
C V (%)
6
4.38
4.22
6.0
5.71
3.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NOS1/nNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NOS1/nNOS in samples. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.
