Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like Protein 2) ELISA Kit
The Rat NFE2L2 (Nuclear Factor Erythroid-derived 2-like protein 2) ELISA Kit is specifically designed for the precise measurement of NFE2L2 levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring the reliability and reproducibility of results for a variety of research applications.NFE2L2 is a critical transcription factor involved in oxidative stress response and regulation of antioxidant genes.
Its dysregulation has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions. By accurately quantifying NFE2L2 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.
Product Name:
Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like Protein 2) ELISA Kit
SKU:
RTES00874
Target:
Rat NFE2L2 (Nuclear Factor, Erythroid Derived 2 Like Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NFE2L2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NFE2L2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NFE2L2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NFE2L2. You can calculate the concentration of Rat NFE2L2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
91-104
88-104
Average (%)
105
97
95
1:4
Range (%)
91-106
83-96
88-101
Average (%)
97
89
93
1:8
Range (%)
93-103
82-94
89-102
Average (%)
98
89
94
1:16
Range (%)
91-102
88-100
81-94
Average (%)
97
93
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.98
139.52
386.82
51.79
143.1
368.46
Standard deviation
2.49
7.09
16.09
2.92
8.57
16.32
C V (%)
5.08
5.08
4.16
5.64
5.99
4.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat NFE2L2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat NFE2L2 in samples. No significant cross-reactivity or interference between Rat NFE2L2 and analogues was observed.
