The Rat MUC2 (Mucin 2) CLIA Kit is specifically designed for the quantitative detection of MUC2 levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and accuracy, providing reliable and reproducible results for a variety of research applications.MUC2 is a key mucin protein involved in maintaining the protective mucus barrier in the gastrointestinal tract.
Abnormalities in MUC2 expression have been linked to various gastrointestinal disorders, such as inflammatory bowel disease and colorectal cancer. Therefore, the Rat MUC2 CLIA Kit serves as a valuable tool for studying the role of MUC2 in these conditions and developing potential therapeutic strategies.
Product Name:
Rat MUC2 (Mucin 2) CLIA Kit
SKU:
RTES00351
Target:
Rat MUC2 (Mucin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MUC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MUC2. You can calculate the concentration of Rat MUC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
104-120
89-103
Average (%)
108
110
95
1:4
Range (%)
102-115
94-106
97-114
Average (%)
108
99
105
1:8
Range (%)
95-113
90-104
89-102
Average (%)
103
97
95
1:16
Range (%)
99-113
103-118
103-120
Average (%)
105
109
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.37
539.01
1623.97
216.93
490.2
1581.67
Standard deviation
26.24
63.39
182.53
24.36
38.28
178.1
C V (%)
12.3
11.76
11.24
11.23
7.81
11.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MUC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MUC2 in samples. No significant cross-reactivity or interference between Rat MUC2 and analogues was observed.
