Rat MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit
The Rat MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MPIF2 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.MPIF2 is a key protein involved in regulating myeloid progenitor cell differentiation, functioning as an inhibitor of cell proliferation.
Its role in immunology and hematopoiesis makes it a valuable biomarker for studying various diseases and conditions related to the immune system and blood cell development.Overall, the Rat MPIF2 CLIA Kit is an essential tool for researchers looking to investigate the role of MPIF2 in different physiological and pathological processes, providing valuable insights for potential therapeutic interventions.
Product Name:
Rat MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit
SKU:
RTES00194
Target:
Rat MPIF2 (Myeloid Progenitor Inhibitory Factor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MPIF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MPIF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MPIF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MPIF2. You can calculate the concentration of Rat MPIF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
95-110
90-101
Average (%)
99
101
95
1:4
Range (%)
100-115
95-108
94-108
Average (%)
109
102
102
1:8
Range (%)
91-104
99-111
89-103
Average (%)
99
105
94
1:16
Range (%)
86-100
98-108
101-116
Average (%)
93
103
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.24
75.97
387.6
34.61
72.97
391.54
Standard deviation
3.95
7.21
26.82
2.98
6.06
40.6
C V (%)
10.33
9.49
6.92
8.61
8.3
10.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MPIF2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MPIF2 in samples. No significant cross-reactivity or interference between Rat MPIF2 and analogues was observed.
