The Rat MMP-1 (Matrix Metalloproteinase-1) CLIA Kit is specifically designed for the quantitative detection of MMP-1 levels in rat serum, plasma, and cell culture supernatants. This highly sensitive and specific kit delivers precise and consistent results, making it ideal for a variety of research applications.MMP-1 is a key enzyme involved in the breakdown of extracellular matrix components, playing a crucial role in tissue remodeling and repair. Abnormal MMP-1 levels have been linked to various diseases, including arthritis, cardiovascular diseases, and cancer, making it a valuable biomarker for understanding disease progression and potential therapeutic interventions.
The Rat MMP-1 CLIA Kit offers researchers a reliable tool for studying MMP-1 dynamics in different biological samples, providing valuable insights into disease mechanisms and therapeutic targets. With its high performance and ease of use, this kit is a valuable asset for any laboratory conducting research on MMP-1 signaling pathways and related diseases.
Product Name:
Rat MMP-1 (Matrix Metalloproteinase 1) CLIA Kit
SKU:
RTES00380
Target:
Rat MMP-1 (Matrix Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-1. You can calculate the concentration of Rat MMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
98-114
97-112
Average (%)
98
106
104
1:4
Range (%)
100-114
93-108
101-116
Average (%)
106
99
109
1:8
Range (%)
93-104
99-115
101-115
Average (%)
98
105
108
1:16
Range (%)
89-104
93-106
96-107
Average (%)
95
100
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.17
312.35
891.64
91.98
325.8
887.84
Standard deviation
10.54
27.33
61.79
10.15
36.82
78.57
C V (%)
10.32
8.75
6.93
11.04
11.3
8.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-1 in samples. No significant cross-reactivity or interference between Rat MMP-1 and analogues was observed.
